Purification and Properties of D-glucose-~-phosphate Dehydrogenase*
نویسندگان
چکیده
n-Glucose-6-phosphate dehydrogenase (Zwischenferment) was discovered by Warburg and Christian in 1931 (1, 2). An active preparation was obtained then from horse erythrocytes and in 1932 from the Lebedev juice made from brewers’ yeast (3). Because of its extreme specificity, this enzyme has become an important analytical tool. Various preparations of it have been described, the first being those of Warburg and Christian (l-5) who found that the active protein could be precipitated from diluted Lebedev juice by acidification with carbon dioxide. We have been able to effect a 5to g-fold purification of the dehydrogenase by this method, but the conditions are not reproducible from one lot of yeast to another. Negelein and Gerischer (6) were able to obtain a preparation about 65-fold purified, in small yield, after an intricate fractionation procedure. More recently Kornberg has described a duplicable procedure which yields a product purified 20-fold and in good yield (7). In this paper we report the further purification of the enzyme and some of its properties.
منابع مشابه
Purification and properties of d-glucose-6-phosphate dehydrogenase.
n-Glucose-6-phosphate dehydrogenase (Zwischenferment) was discovered by Warburg and Christian in 1931 (1, 2). An active preparation was obtained then from horse erythrocytes and in 1932 from the Lebedev juice made from brewers’ yeast (3). Because of its extreme specificity, this enzyme has become an important analytical tool. Various preparations of it have been described, the first being those...
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Glucose-6-phosphate dehydrogenase (G6PD) deficient subjects are vulnerable to oxidative stress. Quercetin, a flavonoids, has been employed as a potent oxygen-free radical scavenger in order to assess the protective effects of quercetin against H2O2-induced oxidative damage in G6PD-deficient and normal human erythrocytes. Erythrocytes of G6PD-deficient (n = 10) and normal (n = 10) subjects were ...
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